Internal and External Coatings and Chemistries:
Coatings, Interior

Interior surface coating of capillary is routinely performed. These modifications fall into three basic categories.

• Wall Coated Open Tubular (WCOT) columns are used routinely in GC. The coating process involves deposition and subsequent cross-linking of the stationary phase. Some chemical bonding to silanol groups is desired, and gives improved temperature stability and reduced bleed. Companies that market GC columns are the primary source for these materials.
• Covalently bonded chemistries find broad use in CE, both as finished columns and polymeric anchors for gel matrices. Bonding reaction schemes are numerous, with silanization using methoxy-, ethoxy-, or chloro-silanes being most common. Grignard reaction schemes are also popular. Often the goal of such coatings is to stabilize, eliminate, or adjust EOF. CE instrument manufacturers and a few after-market suppliers offer these types of coated columns. Polymicro can produce bulk capillary with covalently bonded chemistries. This type of internally coated capillary can be produced on custom basis upon request.
• Dynamic coatings involve the adsorption of specially designed solutions onto the walls of capillary and routine, periodic re-application is required. These typically target the same goals as covalently bonded chemistries.

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